<b>Whole cell patch-clamp recordings of skeletal muscular </b><b>DRG neurons in anesthetized whole </b><b>animal preparations </b>

Whole-Cell Patch-Clamp Recordings

The patch-clamp recording technique, which measures ionic currents under voltage clamp, was designed to study small patches of membranes in which near perfect control of the transmembrane voltage can be achieved readily. The recent application of patch-clamp methodology to the analysis of whole-cell current actually defies many of the original design requirements. Nevertheless, whole-cell recor...

Whole-cell patch-clamp recordings from identified spinal neurons in the zebrafish embryo.

We describe a preparation for obtaining patch-clamp recordings from identified embryonic spinal cord interneurons, motoneurons and sensory neurons in an in vivo zebrafish preparation. This preparation is used to study the spatial and temporal patterns of spontaneous and touch-evoked electrical activity during the initial development of circuitry in the spinal cord. The combination of these phys...

Voltage-clamp measurement of visually-evoked conductances with whole-cell patch recordings in primary visual cortex.

Whole cell patch recordings have been realized in the primary visual cortex of the anesthetized and paralyzed cat, in order to better characterize input resistance and time constant of visual cortical cells in vivo. Measurements of conductance changes evoked by visual stimulation were derived from voltage clamp recordings achieved in continuous mode at two or more different subthreshold holding...

Whole Cell Patch-Clamp Recordings from Lobula plate tangential cells in drosophila melanogaster

The interpretation of current-clamp recordings in the cell-attached patch-clamp configuration.

In these experiments we have investigated the feasibility and accuracy of recording steady-state and dynamic changes in transmembrane potential noninvasively across an intact cell-attached patch using the current-clamp mode of a conventional patch-clamp amplifier. Using an equivalent circuit mimicking simultaneous whole-cell voltage-clamp and cell-attached current-clamp recordings we have defin...